Nikon MICROSCOP E MODE L L-K e INSTRUCTION S NIPPO N KOGAK U K.K . Copy offered by courtesy of Dr. Steven Horii, USA.

Cautio n i n Handlin g an d Maintenanc e • Avoi d touchin g len s surface s wit h th e finger s o r wit h an y roug h material . Fo r dusting , us e a feathe r o r a sof t len s brus h an d the n a .

— 3 NOMENCLATUR E (MODE L LBR-Ke ) Eyepiec e — 4 —.

VARIOU S COMBINATION S Th e Niko n Microscop e Mode l L-K e i s availabl e i n variou s combination s wit h objectives , eyepieces , condensers , eyepiec e tubes , an d stages .

OBJECTIVES , EYEPIECES , CONDENSER S 1 . Objective s a . OBJECTIVE S fo r medical an d biologica l us e Typ e Individua l Magnificatio n Numerica l Foca l Apertur e Lengt h Workin g Distanc e Remark s Achromati c Dr y Oil-immersio n 4 X " lO X 20 X 40 X S40 X NC40 X lOO X SIOO X lOO X 0.

microscop e se t u p i s abou t 500—lOOO X o f th e numerica l apertur e o f th e objectiv e t o b e used . Not e tha t i n photomicrograph y i t i s useles s t o rais e th e magnificatio n beyon d th e resolvin g powe r o f th e emulsio n (usuall y abou t 0.

2 . Mountin g th e Eyepiece s Fo r mounting , simpl y dro p th e eyepiec e int o th e eyepiec e tube . I t i s recommende d t o leav e a n eyepiec e i n plac e eve n whe n i t i s no t i n use , i n orde r t o preven t th e entranc e o f dus t int o th e eyepiec e tube .

smal l ligh t bundles , th e effect s b y diffraction , reflection , refraction , etc. , ma y b e exaggerate d s o tha t fringe s ma y b e see n a t th e imag e edge s whic h ma y likel y induc e misinterpretatio n o f th e image , bu t i t ma y b e effectiv e fo r specia l occassion s (e.

Manipulat e tw o centerin g screw s unti l th e imag e o f th e illuminatio n fiel d diaphrag m come s t o th e center . Yo u nee d no t min d abou t a sligh t deviatio n fro m thi s centerin g cause d b y switchin g ove r t o othe r objective s becaus e thi s wil l no t produc e an y objectionabl e result .

whic h wil l giv e prope r eye-to-len s distance . Fo r thos e wea r eyeglasses , eyecu p shoul d b e screwe d in . 3 . Coars e Focusin g Th e coars e adjustmen t ma y b e ease d o r tightene d b y me.

mediatel y afte r finishin g th e work , clea n of f th e remainin g oi l fro m th e len s usin g a sof t cotto n clot h wette d wit h xylor . Neve r us e alcoho l o r immers e th e to p o f th e objectiv e i n xylor . B e carefu l no t t o us e immersio n oi l tha t ha s bee n thickene d b y age .

2 2 downward , tur n th e rin n o f th e stag e counterclockwise . Fastenin g o f th e floatin g stag e i s necessary , whe n usin g a n attachabl e mechanica l stag e (Fig .

7r O Q • D Q o o.